여려 염색조건이 AgNORs 염색에 미치는 영향

With increasing interest being shown in nucleolar organizer region(NORs) in pathology, it was considered of great importance to evaluate the effect of various conditons on the staining properties. NORs can be demonstrated in paraffin sections by a silver technique(AgNOR method) which was developed from a method used by cytogeneticists for the demonstration of NORs in chromosome spreads. The degree of staining is dependent on the staining conditons including fixation regime employed and results may vary greatly. To evaluate the stainability of AgNORs according to the fixation regime, silver concentration, staining temperature and staining time, the author have done AgNORs staining in the normal liver tissue. Twenty Sprague-Dawley adult female rat were divided into four group; 10% neutral buffered formalin, formal-calcium, Carnoy and Bouin. the rats were sacrified, and the liver removed, fixed by immersion, and processed for light microscopy. The fixative schadules and post-treatments used in this study were based on standard sequences from literature and other staining conditions were determined in our option. We have shown that, m general, formalinbased fixatives(lO% neutral buffered formalin and 10% formal-calcium) give optimal results. Although it badly revealed morphological aspect, Carnoy and Bouin fixative were similar to formalin-based fixation in the AgNORs counting. But, Carnoy fixation have shown significant differences in mean AgNOR count per nucleus when compaired with the other groups(p <0.01). We have shown also that it give adequate results at 20%~30%, 20 OC and 40~ 60 min in silver concentration, staining temperature and staining time, respectively.