2010•Cold Spring Harbor ProtocolsRequires access

Microinjection of RNA and Preparation of Secreted Proteins from Xenopus Oocytes

Hazel Sive, Robert M. Grainger, Richard M. Harland

Abstract

Microinjection of Xenopus oocytes or embryos with messenger RNA (mRNA) is a powerful technique for studying development, including protein expression during development. This protocol describes a method for the preparation of secreted (35)S-labeled proteins following mRNA microinjection into Xenopus oocytes.

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Key concepts: Microinjection, Xenopus, Messenger RNA, Cell biology, RNA, Chemistry, Embryo, Molecular biology