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2010•Cold Spring Harbor ProtocolsRequires access

Microinjection of Xenopus Oocytes

Hazel L. Sive, Robert M. Grainger, Richard M. Harland

Open publisher page 30 citations

Abstract

Xenopus oocytes can be injected with messenger RNA (mRNA) or DNA constructs into the cytoplasm or nucleus, respectively. The cytoplasm can withstand the introduction of up to 50 nL of injected material, and the nucleus can tolerate up to 20 nL. This protocol describes microinjection of both defolliculated and folliculated Xenopus oocytes. Oocytes remain in good condition for a relatively long time, and injections can be continued, if necessary, over a period of days.

About this research paper

What this paper is about

Xenopus oocytes can be injected with messenger RNA (mRNA) or DNA constructs into the cytoplasm or nucleus, respectively. The cytoplasm can withstand the introduction of up to 50 nL of injected material, and the nucleus can tolerate up to 20 nL. This protocol describes microinjection of both defolliculated and folliculated Xenopus oocytes. Oocytes remain in good condition for a relatively long time, and injections can be continued, if necessary, over a period of days.

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OpenAlex reports 30 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Method / approach

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Available abstract

Xenopus oocytes can be injected with messenger RNA (mRNA) or DNA constructs into the cytoplasm or nucleus, respectively. The cytoplasm can withstand the introduction of up to 50 nL of injected material, and the nucleus can tolerate up to 20 nL. This protocol describes microinjection of both defolliculated and folliculated Xenopus oocytes. Oocytes remain in good condition for a relatively long time, and injections can be continued, if necessary, over a period of days.

Key concepts: Microinjection, Xenopus, Cytoplasm, Nucleus, Cell biology, Messenger RNA, RNA, Molecular biology

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