The Role of Cellulase and Polygalacturonase in Abscission of Young and Mature Shamouti Orange Fruits

Abstract During the first eight weeks after setting young citrus fruits gradually lose their ability to abscise at the abscission zone between the stem and the pedicel; in fruits older than eight weeks abscission occurs at the calyx area. The activity of cellulase and polygalacturonase in the two abscission zones was markedly increased before and during abscission, and was localized mainly in the abscission zone. Ethylene accelerated the increase in enzymic activity after an 8‐ to 10‐h lag period; 2,4‐D delayed abscission and enzymic activity when applied during the first 24 h after excision. During this period 2,4‐D also partly suppressed the enhancing effect of ethylene. Early application of cyclo‐heximide inhibited the formation of the enzymes and thus abscission was delayed to a certain extent. Although there are some indications that the relationship between enzymic activity and abscission is a complex one, the data presented indicate that cellulase and polygalacturonase play a significant role in abscission of citrus fruits at various developmental stages. Both enzymes act almost simultaneously and are equally controlled by ethylene and 2,4‐D.