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1984•FEMS Microbiology LettersRequires access

Molecular cloning and physical mapping of the hyd gene ofEscherichia coliK-12

Isao Karube, Masamitsu Tomiyama, Akihiko Kikuchi

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Abstract

The hyd (hydrogenase activity) gene of Escherichia coli K-12 was cloned in a recombinant plasmid designated pEH1 and a restriction endonuclease map for the enzymes EcoRI, BamHI, SalI, and PstI was constructed. This gene was shown to be located at about 6 kilobase pairs (kb) away from the srl operon. Plasmid pEH1 and its derivatives, pEH2 and pEH3 restored hydrogenase activity and fermentative production of hydrogen from glucose in hyd mutant to the wild-type level.

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What this paper is about

The hyd (hydrogenase activity) gene of Escherichia coli K-12 was cloned in a recombinant plasmid designated pEH1 and a restriction endonuclease map for the enzymes EcoRI, BamHI, SalI, and PstI was constructed. This gene was shown to be located at about 6 kilobase pairs (kb) away from the srl operon. Plasmid pEH1 and its derivatives, pEH2 and pEH3 restored hydrogenase activity and fermentative production of hydrogen from glucose in hyd mutant to the wild-type level.

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Available abstract

The hyd (hydrogenase activity) gene of Escherichia coli K-12 was cloned in a recombinant plasmid designated pEH1 and a restriction endonuclease map for the enzymes EcoRI, BamHI, SalI, and PstI was constructed. This gene was shown to be located at about 6 kilobase pairs (kb) away from the srl operon. Plasmid pEH1 and its derivatives, pEH2 and pEH3 restored hydrogenase activity and fermentative production of hydrogen from glucose in hyd mutant to the wild-type level.

Key concepts: EcoRI, Plasmid, Escherichia coli, Operon, Restriction enzyme, Molecular cloning, PstI, Hybrid plasmid

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