A Method to Determine the end Point of Decalcification of Hard Tissue and Bone
Siegfried Müller, J Pleul, Mathias Götz, J Jähnig, Helmut Schädlich
Abstract
Siegfried Müller, J Pleul, Mathias Götz, J Jähnig, Helmut Schädlich
Abstract
A method for decalcification end point determination of mineralized tissue is described. The calcium content of the decalcification solution was determined colorimetrically with a "continuous automatic analyzer" with a high degree of accuracy. The end point method used has been tested on two decalcification methods, 5% nitric acid with or without ultrasonic treatment. The results suggest it is possible to quantitate the decalcification process.
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A method for decalcification end point determination of mineralized tissue is described. The calcium content of the decalcification solution was determined colorimetrically with a "continuous automatic analyzer" with a high degree of accuracy. The end point method used has been tested on two decalcification methods, 5% nitric acid with or without ultrasonic treatment. The results suggest it is possible to quantitate the decalcification process.
Key concepts: Bone decalcification, Nitric acid, Hard tissue, Biomedical engineering, End point, Chemistry, Dentistry, Mathematics