Bone Decalcification Protocol Using 14% EDTA Buffer Solution pH 7.2 - 7.4 v1

This protocol describes the steps required for successful decalcification. Decalcification describes the technique for removing minerals from bone or other calcified tissue so that good-quality paraffin sections can be prepared that will preserve all the essential microscopic elements. Decalcification is carried out after the specimen has been thoroughly fixed and prior to routine processing to paraffin.