ScholarLens Back to search
1981•CarcinogenesisRequires access

Investigation of the effects of the phorbol ester TPA on carcinogen-induced forward to 6-thioguanine-resistance in V79 Chinese hamster cells

Anne R. Kinsella

Open publisher page 18 citations

Abstract

12-O-Tetradecanoyl-phorbol-13-acetate (TPA) was tested for its effect on N'-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induced forward mutagenesis to 6-thioguanine resistance (6TGR) in V79 Chinese hamster cells. Using a replating assay, no effect of TPA on the recovery of 6TGR mutants was observed. Previous reports of TPA enhancement of carcinogen-induced forward mutagenesis using the in situ mutagenesis assay, can be explained in terms of the influence of metabolic co-operation on mutant recovery. TPA is known to eliminate metabolic co-operation, an artefact of the in situ mutagenesis assay system, thus contributing to the enhancement of mutant recovery in TPA treated cultures. This investigation aims to demonstrate that TPA has no influence on mutagenesis per se, positive or negative enhancement of forward mutagenesis being solely a reflection of the mutagenesis assay employed.

About this research paper

What this paper is about

12-O-Tetradecanoyl-phorbol-13-acetate (TPA) was tested for its effect on N'-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induced forward mutagenesis to 6-thioguanine resistance (6TGR) in V79 Chinese hamster cells. Using a replating assay, no effect of TPA on the recovery of 6TGR mutants was observed. Previous reports of TPA enhancement of carcinogen-induced forward mutagenesis using the in situ mutagenesis assay, can be explained in terms of the influence of metabolic co-operation on mutant recovery. TPA is known to eliminate metabolic co-operation, an artefact of the in situ mutagenesis assay system, thus contributing to the enhancement of mutant recovery in TPA treated cultures. This investigation aims to demonstrate that TPA has no influence on mutagenesis per se, positive or negative enhancement of forward mutagenesis being solely a reflection of the mutagenesis assay employed.

Why it matters

OpenAlex reports 18 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

12-O-Tetradecanoyl-phorbol-13-acetate (TPA) was tested for its effect on N'-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induced forward mutagenesis to 6-thioguanine resistance (6TGR) in V79 Chinese hamster cells. Using a replating assay, no effect of TPA on the recovery of 6TGR mutants was observed. Previous reports of TPA enhancement of carcinogen-induced forward mutagenesis using the in situ mutagenesis assay, can be explained in terms of the influence of metabolic co-operation on mutant recovery. TPA is known to eliminate metabolic co-operation, an artefact of the in situ mutagenesis assay system, thus contributing to the enhancement of mutant recovery in TPA treated cultures. This investigation aims to demonstrate that TPA has no influence on mutagenesis per se, positive or negative enhancement of forward mutagenesis being solely a reflection of the mutagenesis assay employed.

Key concepts: Mutagenesis, Chinese hamster, Mutant, Methylnitronitrosoguanidine, Chinese hamster ovary cell, Carcinogen, Hamster, Chemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Investigation of the effects of the phorbol ester TPA on carcinogen-induced forward to 6-thioguanine-resistance in V79 Chinese hamster cells — Research Paper | ScholarLens