2004•European Journal of ImmunogeneticsRequires access

Cloning of rat lymphocyte activation gene‐3 (Lag3; CD223) cDNA and its mRNA expression in rat tissues*

Taehoon Chun, Hyunseok Cho, Y.H. Chung

Abstract

Rat lymphocyte activation gene-3 (Lag3; CD223) cDNA contains an open reading frame (1575 bp) encoding 525 amino acids. Rat Lag3 mRNA transcript was detected as a single species of approximately 2 kb from phytohemagglutinin (PHA)-stimulated lymphocytes. Further analysis revealed that rat Lag3 mRNA was mainly expressed in lymphoid tissues.

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Key concepts: Biology, Complementary DNA, Open reading frame, Molecular biology, Messenger RNA, Gene, Cloning (programming), Molecular cloning