Molecular Cloning of Glycerol-3-phosphate dehydrogenase

In this book, a cloned novel modal has been reported that could be transfer to the plants to produce salt tolerant. Current, book includes the retrieval of gene sequence from database and designing of specific primer. Amplification of selected gene with the help of designed primer and cloning of amplified product through TA cloning strategies. Cloned gene was transformed in E. coli DH5α with the help of pRSET-A vector to express the gene. The results showed that the transformed E. coli tolerate up to certain salinity level. Current investigation clearly suggested an unpretentious approach to resolve the problems such as salt stress. This information would be highly useful for the understanding of molecular cloning of genes responsible for salt tolerance in natural environment.