2012•Advanced materials researchOpen access

Aqueous Two-Phase Extraction of Flavonoids from Artemisia Argyi Leaves Using an Ethanol-Ammonium Sulfate System

Xie Ma, Yang Guo, Li Li

Abstract

The potential of aqueous two phase extraction for the isolation and purification of flavonoids from Artemisia argyi leaves is investigated. Influence of the process parameters such as type of phase forming salts, concentration of salt and ethanol, added salt and temperature was studied. 30% ethanol/18.87% (NH4)2SO4 system was found most suitable for the extraction. The ethanol and salt concentration were found to significantly affect the flavonoids recovery and partition coefficients. The recovery of 93.16% was obtained at the selected process conditions. The results demonstrated the feasibility of aqueous two phase extraction for the isolation and purification of flavonoids.

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Key concepts: Chemistry, Ethanol, Extraction (chemistry), Chromatography, Aqueous solution, Ammonium sulfate, Salt (chemistry), Aqueous two-phase system