2003•The LaryngoscopeRequires access
A Murine Model of Cholesteatoma‐Induced Bone Resorption Using Autologous Dermal Implantation
Holger Sudhoff, Yvonne Liebehenz, Jessica Aschenbrenner, Jae Hyun Jung, H. Hildmann, Stefan Dazert
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Abstract
OBJECTIVE: To investigate a novel murine model for dermal implant-induced osteolysis analogous to bone resorption observed in middle ear cholesteatoma. STUDY DESIGN: Animal experiment. METHODS: We placed autologous dermal implants on the surface of mouse calvaria. The calvaria were examined at days 1, 3, 5, 7, and 14 after implantation by histological study and tartrate-resistant acid phosphatase immunohistochemical processing to detect osteoclasts. RESULTS: Dermal implants showed a significantly increased osteoclast density compared with control samples. The dermal implant tissue remained viable and produced a robust, localized inflammatory osteolytic response on the adjacent calvarial surface. Osteoclasts were predominantly found on the surface of the calvarium with the greatest osteoclast density seen at 5 to 7 days after implantation. CONCLUSION: The mouse model is expected to be a useful tool for investigating the pathogenesis of localized inflammatory bone resorption related to cholesteatoma.