ScholarLens Back to search
1992•Japanese Journal of Cancer ResearchOpen access

Primer‐dependent Amplification of mdr1 mRNA by Polymerase Chain Reaction

Isamu Sugawara, Masatoshi Watanabe, Atsuko Masunaga, Shinji Itoyama, Kazumitsu Ueda

Open full text 17 citations

Abstract

We have developed a sensitive reverse transcription (RT)-polymerase chain reaction (PCR) to evaluate multidrug resistance in various tissues, using seven mdr-specific primer sets. mdr1 mRNA expression was noted in 16/197 samples. However, the apparent degree of mdr1 mRNA expression depended on the sequence of the primers employed. These findings suggest that more than two sets of primers should be used for effective RT-PCR.

About this research paper

What this paper is about

We have developed a sensitive reverse transcription (RT)-polymerase chain reaction (PCR) to evaluate multidrug resistance in various tissues, using seven mdr-specific primer sets. mdr1 mRNA expression was noted in 16/197 samples. However, the apparent degree of mdr1 mRNA expression depended on the sequence of the primers employed. These findings suggest that more than two sets of primers should be used for effective RT-PCR.

Why it matters

OpenAlex reports 17 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

We have developed a sensitive reverse transcription (RT)-polymerase chain reaction (PCR) to evaluate multidrug resistance in various tissues, using seven mdr-specific primer sets. mdr1 mRNA expression was noted in 16/197 samples. However, the apparent degree of mdr1 mRNA expression depended on the sequence of the primers employed. These findings suggest that more than two sets of primers should be used for effective RT-PCR.

Key concepts: Primer (cosmetics), Polymerase chain reaction, Molecular biology, Reverse transcriptase, Messenger RNA, Biology, Reverse transcription polymerase chain reaction, Inverse polymerase chain reaction

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Primer‐dependent Amplification of mdr1 mRNA by Polymerase Chain Reaction — Research Paper | ScholarLens