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Diseased leaf lyophilization: a method for long‐term prevention of loss of virulence in phytopathogenic bacteria

Yoav Bashan, Yaacov Okon

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Abstract

A technique for long‐term preservation of phytopathogenic bacteria is described. It is based on selective multiplication of the bacterial pathogen in host tissue, disinfection of leaf surfaces to reduce contamination, lyophilization of leaves, and storage under dry conditions at ‐ 80°C. With this technique, the pathogenicity of large numbers of the pathogens Pseudomonas syringae pv. tomato and Xanthomonas campestris pv. vesicatoria was maintained for 4 years.

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What this paper is about

A technique for long‐term preservation of phytopathogenic bacteria is described. It is based on selective multiplication of the bacterial pathogen in host tissue, disinfection of leaf surfaces to reduce contamination, lyophilization of leaves, and storage under dry conditions at ‐ 80°C. With this technique, the pathogenicity of large numbers of the pathogens Pseudomonas syringae pv. tomato and Xanthomonas campestris pv. vesicatoria was maintained for 4 years.

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Available abstract

A technique for long‐term preservation of phytopathogenic bacteria is described. It is based on selective multiplication of the bacterial pathogen in host tissue, disinfection of leaf surfaces to reduce contamination, lyophilization of leaves, and storage under dry conditions at ‐ 80°C. With this technique, the pathogenicity of large numbers of the pathogens Pseudomonas syringae pv. tomato and Xanthomonas campestris pv. vesicatoria was maintained for 4 years.

Key concepts: Xanthomonas campestris, Virulence, Pathogenicity, Pathogen, Biology, Pseudomonas syringae, Bacteria, Microbiology

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