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2011•Advanced materials researchOpen access

Extraction, Purification and Antioxidant Activity of Intracellular Polysaccharides from a Strain of Lachnum sp.

Ming Ye, Ru Yue Yuan, Tao Qiu, Jing Min Cai

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Abstract

The microwave method was used to extract Lachnum calyculiforme polysaccharides (LCP). By orthogonal experiments, the optimal conditions of extraction were the microwave temperature was 120 °C, extraction time was 20 min, ratio of water to raw material was 60:1, and the yield of polysaccharides was 12.56 %. LCP-1 was separated and sequentially purified from LCP through Sephadex G-100 column chromatography, which was detected as the homogeneous component. LCP -1 had strong scavenging abilities on O2ˉ•, •OH and DPPH free radicals.

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What this paper is about

The microwave method was used to extract Lachnum calyculiforme polysaccharides (LCP). By orthogonal experiments, the optimal conditions of extraction were the microwave temperature was 120 °C, extraction time was 20 min, ratio of water to raw material was 60:1, and the yield of polysaccharides was 12.56 %. LCP-1 was separated and sequentially purified from LCP through Sephadex G-100 column chromatography, which was detected as the homogeneous component. LCP -1 had strong scavenging abilities on O2ˉ•, •OH and DPPH free radicals.

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Available abstract

The microwave method was used to extract Lachnum calyculiforme polysaccharides (LCP). By orthogonal experiments, the optimal conditions of extraction were the microwave temperature was 120 °C, extraction time was 20 min, ratio of water to raw material was 60:1, and the yield of polysaccharides was 12.56 %. LCP-1 was separated and sequentially purified from LCP through Sephadex G-100 column chromatography, which was detected as the homogeneous component. LCP -1 had strong scavenging abilities on O2ˉ•, •OH and DPPH free radicals.

Key concepts: Sephadex, Polysaccharide, Yield (engineering), Extraction (chemistry), Chromatography, DPPH, Homogeneous, Antioxidant

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