2014•Cold Spring Harbor ProtocolsOpen access

Purification and Culture of Dorsal Root Ganglion Neurons

J. Bradley Zuchero

Abstract

Dorsal root ganglion neurons (DRGs) are sensory neurons that reside in ganglions on the dorsal root of the spinal cord. Here we introduce a method for the acute, prospective purification and culture of DRGs from rodents in a serum-free, defined medium, in the absence of glial cells. This immunopanning-based method facilitates the study of DRG biology and function.

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Key concepts: Dorsal root ganglion, Dorsum, Spinal cord, Ganglion, Sensory system, Anatomy, Function (biology), Neuroscience