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MODEL BULLDING OF PRIMARY CULTURE AND PURIFICATION OF RAT DORSAL ROOT GANGLION NEURONS

Wen Ma

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Abstract

Objective: To establish a feasible method of culture and purification of newborn dorsal root ganglion neurons of rats.Methods: Sufficient dorsal root ganglions(DRG) were obtained by microdissection,then were dissociated with trypsin and EDTA.The dissociated neurons were cultured with DF-12 media and Cytarabine for purification.NSE staining was used to determine the purification percentage of DRG.Results: The isolated and cultured DRG survived and grew in good condition in vitro,and the purified percentage of DRG was over 90%.Conclusion: The method is very feasible in obtaining the massive highly purified newborn dorsal root ganglion neurons of rats.

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What this paper is about

Objective: To establish a feasible method of culture and purification of newborn dorsal root ganglion neurons of rats.Methods: Sufficient dorsal root ganglions(DRG) were obtained by microdissection,then were dissociated with trypsin and EDTA.The dissociated neurons were cultured with DF-12 media and Cytarabine for purification.NSE staining was used to determine the purification percentage of DRG.Results: The isolated and cultured DRG survived and grew in good condition in vitro,and the purified percentage of DRG was over 90%.Conclusion: The method is very feasible in obtaining the massive highly purified newborn dorsal root ganglion neurons of rats.

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Available abstract

Objective: To establish a feasible method of culture and purification of newborn dorsal root ganglion neurons of rats.Methods: Sufficient dorsal root ganglions(DRG) were obtained by microdissection,then were dissociated with trypsin and EDTA.The dissociated neurons were cultured with DF-12 media and Cytarabine for purification.NSE staining was used to determine the purification percentage of DRG.Results: The isolated and cultured DRG survived and grew in good condition in vitro,and the purified percentage of DRG was over 90%.Conclusion: The method is very feasible in obtaining the massive highly purified newborn dorsal root ganglion neurons of rats.

Key concepts: Dorsal root ganglion, Dorsum, Microdissection, Medicine, Staining, Ganglion, Primary culture, In vitro

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