Reversible Inhibition of Tomato Fruit Senescence by Antisense RNA
Paul W. Oeller, Lu Min-Wong, Loverine P. Taylor, Deborah A. Pike, Anasios A Theologis
Abstract
Paul W. Oeller, Lu Min-Wong, Loverine P. Taylor, Deborah A. Pike, Anasios A Theologis
Abstract
Ethylene controls fruit ripening. Expression of antisense RNA to the rate-limiting enzyme in the biosynthetic pathway of ethylene, 1-aminocyclopropane-1-carboxylate synthase, inhibits fruit ripening in tomato plants. Administration of exogenous ethylene or propylene reverses the inhibitory effect. This result demonstrates that ethylene is the trigger and not the by-product of ripening and raises the prospect that the life-span of plant tissues can be extended, thereby preventing spoilage.
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Ethylene controls fruit ripening. Expression of antisense RNA to the rate-limiting enzyme in the biosynthetic pathway of ethylene, 1-aminocyclopropane-1-carboxylate synthase, inhibits fruit ripening in tomato plants. Administration of exogenous ethylene or propylene reverses the inhibitory effect. This result demonstrates that ethylene is the trigger and not the by-product of ripening and raises the prospect that the life-span of plant tissues can be extended, thereby preventing spoilage.
Key concepts: Ripening, Ethylene, Limiting, RNA, Enzyme, Senescence, Chemistry, Biochemistry