Michael R. Green, Joseph Sambrook
Abstract
This protocol describes steps for the preparation and running of agarose gels and for staining and visualization of DNA in gels using three dyes: ethidium bromide, SYBR Gold, and SYBR Green 1.
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This protocol describes steps for the preparation and running of agarose gels and for staining and visualization of DNA in gels using three dyes: ethidium bromide, SYBR Gold, and SYBR Green 1.
Key concepts: Ethidium bromide, Agarose, Agarose gel electrophoresis, Gel electrophoresis of nucleic acids, SYBR Green I, Chemistry, Color marker, Chromatography