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2013•Advanced materials researchOpen access

The Effect of Ammonium Chloride on the Apoptosis Induced by Cisplatin in Human Lung Cancer A549 Cells

Ye Xu, Ning Wang, Yan Ding, Yang Yu, Shi Bing Liu, Xiao Jun Wang, Zhi Xin Li

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Abstract

The aim of this study is to investigate the effects and mechanism of ammonium chloride (NH4Cl) on the apoptosis induced by cisplatin in human lung cancer A549 cells. MTT assay was used to detect the state of cell growth. The expressions of apoptosis related proteins were detected by Laser scanning confocal microscopy and western blot. MTT tests showed that the non-toxic dose of NH4Cl could increase the inhibition rate of A549 cells induced by cisplatin. The expression of active caspase-3 in cells treated with non-toxic dose of NH4Cl combined with cisplatin was higher than that in cells treated with cisplatin alone. Western blot analysis showed that the expressions of apoptosis related proteins were significantly increased in the combination group. These results indicate that NH4Cl can enhance the cell apoptosis induced by cisplatin in A549 cells.

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What this paper is about

The aim of this study is to investigate the effects and mechanism of ammonium chloride (NH4Cl) on the apoptosis induced by cisplatin in human lung cancer A549 cells. MTT assay was used to detect the state of cell growth. The expressions of apoptosis related proteins were detected by Laser scanning confocal microscopy and western blot. MTT tests showed that the non-toxic dose of NH4Cl could increase the inhibition rate of A549 cells induced by cisplatin. The expression of active caspase-3 in cells treated with non-toxic dose of NH4Cl combined with cisplatin was higher than that in cells treated with cisplatin alone. Western blot analysis showed that the expressions of apoptosis related proteins were significantly increased in the combination group. These results indicate that NH4Cl can enhance the cell apoptosis induced by cisplatin in A549 cells.

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Available abstract

The aim of this study is to investigate the effects and mechanism of ammonium chloride (NH4Cl) on the apoptosis induced by cisplatin in human lung cancer A549 cells. MTT assay was used to detect the state of cell growth. The expressions of apoptosis related proteins were detected by Laser scanning confocal microscopy and western blot. MTT tests showed that the non-toxic dose of NH4Cl could increase the inhibition rate of A549 cells induced by cisplatin. The expression of active caspase-3 in cells treated with non-toxic dose of NH4Cl combined with cisplatin was higher than that in cells treated with cisplatin alone. Western blot analysis showed that the expressions of apoptosis related proteins were significantly increased in the combination group. These results indicate that NH4Cl can enhance the cell apoptosis induced by cisplatin in A549 cells.

Key concepts: Cisplatin, Apoptosis, A549 cell, Western blot, MTT assay, Chemistry, Ammonium chloride, Molecular biology

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The Effect of Ammonium Chloride on the Apoptosis Induced by Cisplatin in Human Lung Cancer A549 Cells — Research Paper | ScholarLens