ScholarLens Back to search
1975•European Journal of BiochemistryRequires access

Readenylation of Polyadenylate‐Free Globin Messenger RNA Restores Its Stability in vivo

Georges Huez, Gérard Marbaix, E. Hubert, Y. Cleuter, M Leclercq, Hubert Chantrenne, René Devos, Hermona Soreq, Uri Nudel, Uriel Z. Littauer

Open publisher page 72 citations

Abstract

Using an ATP:RNA adenyltransferase from Escherichia coli, a polyadenylic sequence was resynthesized onto rabbit globin mRNA from which the poly (A) segment had been previously removed. Conditions for obtaining a homogenous reconstituted globin mRNA preparation containing 30 adenylic residues per message molecule were determined. The reconstituted globin mRNA was microinjected into Xenopus laevis oocytes. Its stability was very similar to that of native mRNA.

About this research paper

What this paper is about

Using an ATP:RNA adenyltransferase from Escherichia coli, a polyadenylic sequence was resynthesized onto rabbit globin mRNA from which the poly (A) segment had been previously removed. Conditions for obtaining a homogenous reconstituted globin mRNA preparation containing 30 adenylic residues per message molecule were determined. The reconstituted globin mRNA was microinjected into Xenopus laevis oocytes. Its stability was very similar to that of native mRNA.

Why it matters

OpenAlex reports 72 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Using an ATP:RNA adenyltransferase from Escherichia coli, a polyadenylic sequence was resynthesized onto rabbit globin mRNA from which the poly (A) segment had been previously removed. Conditions for obtaining a homogenous reconstituted globin mRNA preparation containing 30 adenylic residues per message molecule were determined. The reconstituted globin mRNA was microinjected into Xenopus laevis oocytes. Its stability was very similar to that of native mRNA.

Key concepts: Globin, Messenger RNA, Xenopus, RNA, Molecular biology, Escherichia coli, In vivo, Biology

Related papers

Related paper 1Related paper 2
Back to paper searchBrowse research topicsOriginal source
Readenylation of Polyadenylate‐Free Globin Messenger RNA Restores Its Stability in vivo — Research Paper | ScholarLens