Separation of Calcium-binding Protein Derived from Enzymatic Hydrolysates of Cheese Whey Protein

This study was carried out to separate the calcium-binding protein derived from enzymatic hydrolysates of cheese whey protein.CWPs (cheese whey protein) heated for 10 min at 100°C were hydrolyzed by trypsin, papain W-40, protease S, neutrase 1.5 and pepsin, and then properties of hydrolysates, separation of calcium-binding protein and analysis of calcium-binding ability were investigated.The DH (degree of hydrolysis) and NPN (non protein nitrogen) of heated-CWP hydrolysates by commercial enzymes were higher in trypsin than those of other commercial enzymes.In the result of SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis), β-LG and α-LA in trypsin hydrolysates were almost eliminated and the molecular weight of peptides derived from trypsin hydrolysates were smaller than 7 kDa.In the RP-HPLC (reverse phase HPLC) analysis, α-LA was mostly eliminated, but β-LG was not affected by heat treatment and the RP-HPLC patterns of trypsin hydrolysates were similar to those of SDS-PAGE.In ion exchange chromatography, trypsin hydrolysates were shown to peak from 0.25 M NaCl and 0.5 M NaCl, and calcium-binding ability is associated with the large peak, which was eluted at a 0.25 M NaCl gradient concentration.Based on the results of this experiment, heated-CWP hydrolysates by trypsin were shown to have calcium-binding ability.(Asian-Aust.