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Determination of oxysophocarpine in different organs of Sophora viciifolia by HPLC.

Yufei Li, WU San-qiao, Yanlong Wang, Liu Rong-tang, Xinsheng Li

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Abstract

This paper established an analytical method to determine the content of oxysophocarpine in Sophora viciifolia by HPLC.ZORBAX-C18(150 mm×4.6 mm,5 μm)Column and 0.04 mol/L H3PO4(pH=1.8)-CH3OH(90∶10) mobile phase were used.The identification wave length was 220 nm and column temperature was 20 ℃.The result showed that the buds were the main accumulation organs of oysophocarpine(2.114%).The contents of oxysophocarpine in other organs such as young fruit,seeds,flowers,leaves,one year growing stem and perennial stem was 1.735%、1.651%、1.615%、0.540%、0.244% and 0.045%,respectively.This result is helpful for S.viciifolia resources development and ecology protection.

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What this paper is about

This paper established an analytical method to determine the content of oxysophocarpine in Sophora viciifolia by HPLC.ZORBAX-C18(150 mm×4.6 mm,5 μm)Column and 0.04 mol/L H3PO4(pH=1.8)-CH3OH(90∶10) mobile phase were used.The identification wave length was 220 nm and column temperature was 20 ℃.The result showed that the buds were the main accumulation organs of oysophocarpine(2.114%).The contents of oxysophocarpine in other organs such as young fruit,seeds,flowers,leaves,one year growing stem and perennial stem was 1.735%、1.651%、1.615%、0.540%、0.244% and 0.045%,respectively.This result is helpful for S.viciifolia resources development and ecology protection.

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Available abstract

This paper established an analytical method to determine the content of oxysophocarpine in Sophora viciifolia by HPLC.ZORBAX-C18(150 mm×4.6 mm,5 μm)Column and 0.04 mol/L H3PO4(pH=1.8)-CH3OH(90∶10) mobile phase were used.The identification wave length was 220 nm and column temperature was 20 ℃.The result showed that the buds were the main accumulation organs of oysophocarpine(2.114%).The contents of oxysophocarpine in other organs such as young fruit,seeds,flowers,leaves,one year growing stem and perennial stem was 1.735%、1.651%、1.615%、0.540%、0.244% and 0.045%,respectively.This result is helpful for S.viciifolia resources development and ecology protection.

Key concepts: Perennial plant, High-performance liquid chromatography, Botany, Biology, Stem-and-leaf display, Column (typography), Horticulture, Chromatography

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Determination of oxysophocarpine in different organs of Sophora viciifolia by HPLC. — Research Paper | ScholarLens