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1994•Journal of Herbs Spices & Medicinal PlantsOpen access

Micropropagation ofMaytenus aquifoliumMartius

Ana Maria Soares Pereira, José Roberto Môro, Rita M.M. Cerdeira, Suzelei de Castro França

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Abstract

Axillary buds of Maytenus aquifolium were cultured in Murashige and Skoog (MS) medium with growth regulators to induce plantlet formation. Shoot proliferation induced in MS medium supplemented 3 mg/l with 6-benzylaminopurine (BAP) was greater than in MS medium with kinetin at the same concentration. Plantlet height was also affected by both type and concentration of cytokinin. Addition of 0.2 mg/l of indole-3-acetic acid (IAA) to the medium contributed to an increase in plantlet height. Rootless plantlets were rooted successfully in vitro or ex vitro.

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What this paper is about

Axillary buds of Maytenus aquifolium were cultured in Murashige and Skoog (MS) medium with growth regulators to induce plantlet formation. Shoot proliferation induced in MS medium supplemented 3 mg/l with 6-benzylaminopurine (BAP) was greater than in MS medium with kinetin at the same concentration. Plantlet height was also affected by both type and concentration of cytokinin. Addition of 0.2 mg/l of indole-3-acetic acid (IAA) to the medium contributed to an increase in plantlet height. Rootless plantlets were rooted successfully in vitro or ex vitro.

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Available abstract

Axillary buds of Maytenus aquifolium were cultured in Murashige and Skoog (MS) medium with growth regulators to induce plantlet formation. Shoot proliferation induced in MS medium supplemented 3 mg/l with 6-benzylaminopurine (BAP) was greater than in MS medium with kinetin at the same concentration. Plantlet height was also affected by both type and concentration of cytokinin. Addition of 0.2 mg/l of indole-3-acetic acid (IAA) to the medium contributed to an increase in plantlet height. Rootless plantlets were rooted successfully in vitro or ex vitro.

Key concepts: Plantlet, Kinetin, Axillary bud, Micropropagation, Murashige and Skoog medium, Cytokinin, Botany, Shoot

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