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Rapid Detection of Yeast in Orange Juice by Enzyme-linked Immunosorbent Assay.

Motonobu Yoshida, Hisao Maeda, Yasushi Ifuku

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Abstract

Antisera against native yeast of 6 species, Candida intermedia, C. parapsilosis, C. guilliermondii, C. lambica, Crytococcus laurentii, and Rhodotorula rubra, were prepared for use as probes in an enzyme-linked immunosorbent assay (ELISA).These antisera reacted with yeast cell surface antigens that are thought to consist of protein moieties.The cross-reactivities between the antisera and yeast of ll species were investigated by immunofluorescent and immunoblotting methods.It was shown that ELISAusing the antiserum against C. intermedia or C. parapsilosis, is a valuable meansof detecting yeast in orange juice.Yeast of more than 103 were capable of being detected by ELISA.

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Antisera against native yeast of 6 species, Candida intermedia, C. parapsilosis, C. guilliermondii, C. lambica, Crytococcus laurentii, and Rhodotorula rubra, were prepared for use as probes in an enzyme-linked immunosorbent assay (ELISA).These antisera reacted with yeast cell surface antigens that are thought to consist of protein moieties.The cross-reactivities between the antisera and yeast of ll species were investigated by immunofluorescent and immunoblotting methods.It was shown that ELISAusing the antiserum against C. intermedia or C. parapsilosis, is a valuable meansof detecting yeast in orange juice.Yeast of more than 103 were capable of being detected by ELISA.

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Available abstract

Antisera against native yeast of 6 species, Candida intermedia, C. parapsilosis, C. guilliermondii, C. lambica, Crytococcus laurentii, and Rhodotorula rubra, were prepared for use as probes in an enzyme-linked immunosorbent assay (ELISA).These antisera reacted with yeast cell surface antigens that are thought to consist of protein moieties.The cross-reactivities between the antisera and yeast of ll species were investigated by immunofluorescent and immunoblotting methods.It was shown that ELISAusing the antiserum against C. intermedia or C. parapsilosis, is a valuable meansof detecting yeast in orange juice.Yeast of more than 103 were capable of being detected by ELISA.

Key concepts: Antiserum, Yeast, Candida parapsilosis, Orange (colour), Chemistry, Enzyme, Biochemistry, Orange juice

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Rapid Detection of Yeast in Orange Juice by Enzyme-linked Immunosorbent Assay. — Research Paper | ScholarLens