2010•Protein Expression and PurificationRequires access

A strategy for high-level expression of a single-chain variable fragment against TNFα by subcloning antibody variable regions from the phage display vector pCANTAB 5E into pBV220

Tao Yang, Lijun Yang, Weiran Chai, Ren‐Ke Li, Jun Xie, Bo Niu

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Key concepts: Subcloning, Single-chain variable fragment, Molecular biology, Recombinant DNA, Escherichia coli, Phage display, Affinity chromatography, Inclusion bodies

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A strategy for high-level expression of a single-chain variable fragment against TNFα by subcloning antibody variable regions from the phage display vector pCANTAB 5E into pBV220 — Research Paper | ScholarLens