2002•Biosensors and BioelectronicsOpen access

A generic strategy for subcloning antibody variable regions from the scFv phage display vector pCANTAB 5 E into pASK85 permits the economical production of Fab fragments and leads to improved recombinant immunoglobulin stability

Karl Kramer

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Key concepts: Subcloning, Single-chain variable fragment, Recombinant DNA, Antibody, Chemistry, Molecular biology, Periplasmic space, Phage display

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A generic strategy for subcloning antibody variable regions from the scFv phage display vector pCANTAB 5 E into pASK85 permits the economical production of Fab fragments and leads to improved recombinant immunoglobulin stability — Research Paper | ScholarLens