1994•FEMS Microbiology LettersRequires access

Subdivision ofSalmonella enteritidisPT 4 by pulsed-field gel electrophoresis: Potential for epidemiological surveillance

N.G. Powell, E. J. Threlfall, H. Chart, B. Rowe

Abstract

Using pulsed-field gel electrophoresis (PFGE), nine profile types were identified in XbaI digests of a panel of strains of Salmonella enteritidis phage type 4 (PT 4) isolated in England and Wales between 1967 and 1992. When applied to 39 isolates from humans, chickens, poultry products and feed, strains belonging to pulsed-field profile (PFP) 1 predominated. DNA-DNA hybridization studies demonstrated that a band of approximately 57 kb was associated with the presence of the 38 MDa S. enteritidis 'serovar-specific' plasmid. It is concluded that PFGE provides a method for discriminating strains of S. enteritidis PT 4 suitable for epidemiological investigations.

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Key concepts: Salmonella enteritidis, Pulsed-field gel electrophoresis, Salmonella, Microbiology, Epidemiological surveillance, Epidemiology, Biology, Medicine