Basic study of spatial resolution measurement for autoradiography systems

A spatial resolution of real-time bioradiography system was closely measured using PDMS (polydimethyl siloxane) microfluidic chips with microchannels down to 20um wide. We measured the spatial resolution by comparing line width of a chemiluminescence image and a beta-ray image. Both images were obtained using identical microchannel. Using our method, a spatial resolution can be measured within an equal condition to actual observation. In addition, the spatial resolution was improved upto 20um FWHM(Full Width at Half Maximum), which is 50 times better than that of conventional method. Owing this method, we experimentally confirmed that b-ray scattering degrades the spatial resolution of the β-ray image.