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Purification of Rat Gamma Globulin and the Production of a Specific Antirat Gamma Globulin Serum

E. D. Exum, Robert T. Bowser

Abstract

A method for the isolation of rat 7S gamma globulin based on multiple adsorptions with DEAE-Sephadex A-50 was presented. The separation of rat 7S globulin requires a procedure modified from the one used for humans in terms of the buffer pH, and the number of adsorptions. Ultracentrifugal analysis indicates the resulting fraction is a single component. In addition, immunoelectrophoresis of the fraction when tested against antirat serum confirms the homogeneity of this 7S fraction. A monospecific antiserum to rat 7S gamma globulin was produced. This antiserum is free of reaction with all other serum components when subjected to immunoelectrophoresis against normal rat serum.

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Key concepts: Antiserum, Immunoelectrophoresis, Gamma globulin, Sephadex, Chromatography, Fraction (chemistry), Globulin, Chemistry