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1968•Scandinavian Journal of HaematologyRequires access

Antibody to Transcobalamin II in Patients Treated with Long Acting Vitamin B12 Preparations

H. Olesen, Ben Lin Hom, Michael Schwartz

Open publisher page 35 citations

Abstract

57Co‐labelled vitamin B12 bound to transcobalamin I, transcobalamin II or non‐protein bound, has been mixed with serum from patients with pernicious anaemia treated with long acting vitmain B12 preparations. By paper electrophoresis, immunoelectrophoresis and Sephadex G‐200 gel filtration, an antibody of the γG‐globulin type reacting with transcobalamin II was demonstrated. By immunoelectrophoresis the antibody was demonstrated in 8 of 19 patients. Gel filtration experiments showed that the affinity of the antibody to TC II is very sensitive to changes in pH, ionic strength and temperature.

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What this paper is about

57Co‐labelled vitamin B12 bound to transcobalamin I, transcobalamin II or non‐protein bound, has been mixed with serum from patients with pernicious anaemia treated with long acting vitmain B12 preparations. By paper electrophoresis, immunoelectrophoresis and Sephadex G‐200 gel filtration, an antibody of the γG‐globulin type reacting with transcobalamin II was demonstrated. By immunoelectrophoresis the antibody was demonstrated in 8 of 19 patients. Gel filtration experiments showed that the affinity of the antibody to TC II is very sensitive to changes in pH, ionic strength and temperature.

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OpenAlex reports 35 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

57Co‐labelled vitamin B12 bound to transcobalamin I, transcobalamin II or non‐protein bound, has been mixed with serum from patients with pernicious anaemia treated with long acting vitmain B12 preparations. By paper electrophoresis, immunoelectrophoresis and Sephadex G‐200 gel filtration, an antibody of the γG‐globulin type reacting with transcobalamin II was demonstrated. By immunoelectrophoresis the antibody was demonstrated in 8 of 19 patients. Gel filtration experiments showed that the affinity of the antibody to TC II is very sensitive to changes in pH, ionic strength and temperature.

Key concepts: Sephadex, Immunoelectrophoresis, Size-exclusion chromatography, Antibody, Chemistry, Ionic strength, Chromatography, Vitamin

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