2010•Advanced materials researchOpen access

Plasmid Fingerprinting Profiles of Chicken-Derived Pathogenic Strains of Escherichia coli

Chun Guang Wang, Tie Zhang, Xing Hua Zhao

Abstract

Plasmid DNA was extracted from all 38 strains of chicken Escherichia coli by alkaline lysis method. The agarose gel electrophoresis showed 23 plasmid patterns. The strains from the same source had the same, similar, or quite different plasmid profiles, while those from different sources generally did not have the same plasmid profiles. Therefore, the plasmid profiles can provide basic materials for epidemiological investigation on chicken Escherichia coli at molecular level.

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Key concepts: Plasmid, Escherichia coli, Alkaline lysis, Plasmid preparation, Agarose gel electrophoresis, Biology, Agarose, Lysis