Technology Optimization on Preparation of Peanut Polypeptide Using Alcalase

In order to exploit and utilize the peanut protein resource and produce high additional value protein products, the preparation technology of peanut polypeptide from peanut protein isolate was investigated using Alcalase. The effects of influencing factors, enzyme dosage, substrate mass fraction, hydrolysis temperature, hydrolysis time and pH value, on hydrolysis was studied during the process of Alcalase hydrolyzing peanut protein isolate by single factor experiment and response surface methodology central composite design. The regression model for the relationship between the influencing factors and the soluble nitrogen mass concentration was established. The optimum parameters for Alcalase enzymatic reaction were obtained as follows: pH value 8.0, enzyme dosage 4762U/g substrate, substrate mass fraction 5%, hydrolysis temperature 50°C, hydrolysis time 180min, and then the soluble nitrogen mass concentration reached to 16.28mg/mL.