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2013•Advanced materials researchOpen access

Protective Effects of Gynostemma pentaphyllum Makino Polysaccharide on Alcoholic Hepatic Injuries

Shu Liang Song, Zeng Xiao, Hao Liang, Yun Shan Wang, Aiguo Ji

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Abstract

Crude Gynostemma pentaphyllum Makino polysaccharide was extracted by water extraction and ethanol precipitation. After purification by depectinization and decolourisation, polysaccharide GPS-3 was obtained by purification using Q Sepharose Fast Flow and Superdex 200 columns. Furthermore, the protective effects of GPS-3 on alcoholic hepatic injuries of mice were studied. Results showd that GPS-3 had significant protective effects on alcoholic hepatic injuries.

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What this paper is about

Crude Gynostemma pentaphyllum Makino polysaccharide was extracted by water extraction and ethanol precipitation. After purification by depectinization and decolourisation, polysaccharide GPS-3 was obtained by purification using Q Sepharose Fast Flow and Superdex 200 columns. Furthermore, the protective effects of GPS-3 on alcoholic hepatic injuries of mice were studied. Results showd that GPS-3 had significant protective effects on alcoholic hepatic injuries.

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Available abstract

Crude Gynostemma pentaphyllum Makino polysaccharide was extracted by water extraction and ethanol precipitation. After purification by depectinization and decolourisation, polysaccharide GPS-3 was obtained by purification using Q Sepharose Fast Flow and Superdex 200 columns. Furthermore, the protective effects of GPS-3 on alcoholic hepatic injuries of mice were studied. Results showd that GPS-3 had significant protective effects on alcoholic hepatic injuries.

Key concepts: Gynostemma pentaphyllum, Polysaccharide, Extraction (chemistry), Precipitation, Ethanol, Chemistry, Chromatography, Ethanol precipitation

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