Separation,Purification and Antioxidation Character of the Ganoderma lucidum Polysaccharides

Two deproteinization methods,Sevag and HCl,were compared;and amount of polysaccharide precipitated by different concentrations of ethanol,antioxidant activity of Ganoderma lucidum polysaccharides(GLP) were studied.The results showed that the methods of Sevag and HCl could both remove the protein which in the Ganoderma lucidum polysaccharide extraction,but removing rate and polysaccharide loss rate differs,Sevag method polysaccharide loss rate was 36.08%,and protein removal rate was 82.79%.The loss of polysaccharide of HCl method was 14.41%,and protein removal rate was 41.17%.The ethanol precipitation GLP study showed that 40% ethanol,65% ethanol and 80% ethanol's precipitation amount GLP were GL1:GL2:GL3=0.41:0.42:0.17;the higher the concentration of polysaccharide the stronger removing ability of hydroxyl radicals and superoxide anion,precipitation of the polysaccharide concentration in conditions of antioxidant activity was:GL3GL1GL2.