Estimation of meiosis and sporulation efficiencies in the fission yeast by ascus analysis

SUMMARY Populations of linear asci are classified according to the number of spores in an ascus. The resultant five numerical classes are further classified into ten spatial classes according to the arrangement of the spores in an ascus and, by inference, into ten historical classes according to the number and origins of failures during the developmental process. An analysis of the observed frequencies of numerical classes allows derivation of the efficiencies of the first meiotic division, the second meiotic division and sporulation in a fission yeast. The analytical method may be useful in locating the site of action of sporulation inhibitors and in identifying meiosis mutants from sporulation mutants.