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2011•Advanced materials researchRequires access

Screening of Yeast with High-Yield Single Cell Protein and Preliminary Optimization of its Culture Codition

Min Shi, Ming Zheng Liang, Hong Yu Liang, Mei Li, An Ping Liao

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Abstract

The protein content of Rhodotorula rubra 2.1817,Candida tropicalis 2.1775 and Geotrichum candidum 2.1175 were assayed by Coomassie brilliant blue methods. Screening the high-yield SCP strains.Then,the cultur condition of the strains were optimizated by single-factor test.It is shown that the high-yield SCP strains was Candida tropicalis 2.1775,the protein content was 32.46%.The optimal factors: inoculum size 10% (V/V),temperature 30°C,shaking 200 rpm,culture time 5 days.

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What this paper is about

The protein content of Rhodotorula rubra 2.1817,Candida tropicalis 2.1775 and Geotrichum candidum 2.1175 were assayed by Coomassie brilliant blue methods. Screening the high-yield SCP strains.Then,the cultur condition of the strains were optimizated by single-factor test.It is shown that the high-yield SCP strains was Candida tropicalis 2.1775,the protein content was 32.46%.The optimal factors: inoculum size 10% (V/V),temperature 30°C,shaking 200 rpm,culture time 5 days.

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Available abstract

The protein content of Rhodotorula rubra 2.1817,Candida tropicalis 2.1775 and Geotrichum candidum 2.1175 were assayed by Coomassie brilliant blue methods. Screening the high-yield SCP strains.Then,the cultur condition of the strains were optimizated by single-factor test.It is shown that the high-yield SCP strains was Candida tropicalis 2.1775,the protein content was 32.46%.The optimal factors: inoculum size 10% (V/V),temperature 30°C,shaking 200 rpm,culture time 5 days.

Key concepts: Geotrichum, Candida tropicalis, Yeast, Yield (engineering), Coomassie Brilliant Blue, Microbiology, Biology, Rhodotorula

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