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Avidin-Biotin Immunoperoxidase Technique: An Accelerated Procedure

Lisa M. Roth, Anthony Marchand

Open publisher page 1 citations

Abstract

Modifications of the Avidin-Biotin immunoperoxidase procedure (AB) for visual identification of tissueantigens are described. The alterations in the technique result in a 75 % reduction of processing time while at the same time improving sensitivity and enhancing tissue morphology. The use of this rapid technique allows this procedure to be utilized as a routine diagnostic method with a minimal expenditure in terms of labor and equipment.

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What this paper is about

Modifications of the Avidin-Biotin immunoperoxidase procedure (AB) for visual identification of tissueantigens are described. The alterations in the technique result in a 75 % reduction of processing time while at the same time improving sensitivity and enhancing tissue morphology. The use of this rapid technique allows this procedure to be utilized as a routine diagnostic method with a minimal expenditure in terms of labor and equipment.

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Available abstract

Modifications of the Avidin-Biotin immunoperoxidase procedure (AB) for visual identification of tissueantigens are described. The alterations in the technique result in a 75 % reduction of processing time while at the same time improving sensitivity and enhancing tissue morphology. The use of this rapid technique allows this procedure to be utilized as a routine diagnostic method with a minimal expenditure in terms of labor and equipment.

Key concepts: Immunoperoxidase, Avidin, Biotin, Identification (biology), Biomedical engineering, Pathology, Computer science, Chemistry

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