A New Paraffin Method for the Combined Staining of Myelin and Glial FiBERs*†

A new paraffin method (PFG stain) is described for the differential staining of myelin and glial fibers, utilizing phloxine B, fast green FCF, and gallocyanin. It offers the advantage of providing a strong, intense staining of both of these elements, at the same time yielding an effective color contrast. The method is easily carried out, gives consistent results, and no step requires microscopic control. Although one step of the technic involves overnight staining, this can be altered to allow completion within one day if desired, with almost as satisfactory results. A myelin-cell modification is also described which stains only myelin sheaths, cell nuclei, and Nissl substance.