Comparison of a whole blood test and an ELISA for the of the antigens of Dirofilaria immitis agglutination detection in dogs

To compare the usefulness of two commercial tests for detecting the antigens of Dirofilaria immitis in dogs, one based on whole-blood agglutination (WBA) and the other on ELISA, 100 stray dogs from North Taiwan were tested before necropsy. Of the 53 dogs found to contain D. immitis at necropsy, which had a mean (S.D.) burden of 8.2 (10.6) worms/dog, 45 were found to be positive by WBA and 47 by ELISA. All the false negatives were dogs with very low worm burdens. Although the ELISA was more sensitive (83.9% v. 71.7%) and specific (100% v. 85.1%) than the WBA, the latter is simpler to use and less time-consuming. In terms of their general use for diagnosis of canine heartworm, there seems little to choose between the two tests. The false negatives observed with both tests are not likely to be a problem as they represent dogs with worm burdens which are probably too low to cause significant clinical manifestations or pathology. As the positive predictive value of the WBA test declines dramatically with prevalence of infection, this test may not be suitable for detecting D. immitis in canine populations in which heartworm infection is rare.