1980•Journal of Histochemistry & CytochemistryOpen access

A reliable Feulgen-acriflavine-SO2 staining procedure for quantitative DNA measurements.

Hans J. Tanke, E M van Ingen

Abstract

Feulgen-acriflavine-SO2 staining was performed on chicken erythrocytes and rat liver cells under different cytochemical conditions. The obtained results were compared to conventional Feulgen-pararosaniline-SO2 staining by means of scanning cytophotometry and microfluorometry. It was found that if acid ethanol rinsing was included in the Feulgen-acriflavine-SO2 procedure, both Feulgen procedures were fully comparable in specificity and quantitative aspects.

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Available abstract

Key concepts: Acriflavine, Feulgen stain, Staining, Chemistry, DNA, Biochemistry, Molecular biology, Biology