Comparison among three procedures of Feulgen staining

Objective To develop a quick Feulgen staining and try its application in cell nuclei DNA content measurement.Methods Smears of liver cell suspension were obtained from ten healthy mice.The specimens were divided into three groups,stained with quick Feulgen staining,modified one and traditional one,respectively.DNA content of intact hepatocyte nuclei was analyzed by TIGER cell image analysis system.The results were compared among different groups.Results It took only 15 minutes to finish the quick Feulgen staining.Results with the quick Feulgen staining was satisfactory,that structure of cell nuclei was clear and were stained well,the CV for DNA content of hepatocyte nuclei with same DNA content ploidy was less than 10 percent among different mice hepatocyte nuclei stained using the same staining procedure, i.e.CV_((quick))CV_((modified))CV_((traditional)),the nuclear DNA content of hepatocytes with the same DNA ploidy from the same mice stained by different staining procedures was different,i.e.AIOD_((quick))AIOD_((modified))AIOD_((traditional)),the AIOD ratio among 2c,4c and 8c in each group was close to 2 and 4.Conclusion The quick Feulgen staining is better then the others.It is applicable in image analysis system to measure and analyze the cell nuclei DNA content and its ploidy.