1973•Proceedings of the National Academy of SciencesOpen access

Purification of Mouse Interferon by Affinity Chromatography on a Solid-Phase Immunoadsorbent

Jean D. Sipe, Jaqueline De Maeyer‐Guignard, Bernard Fauconnier, Edward De Maeyer

Abstract

A solid-phase immunoadsorbent capable of binding mouse interferon has been prepared. Starting from crude tissue-culture material, interferon could be purified 1990 times in a single step of affinity chromatography. Overall recovery ranged from 55 to 103% with tissue culture and mouse-brain interferon; however, only 5% was obtained with Sendai virus-induced interferon from mouse serum.

About this research paper

What this paper is about

Why it matters

OpenAlex reports 35 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Sendai virus, Interferon, Affinity chromatography, Chemistry, Tissue culture, Chromatography, Molecular biology, Virus