A preparative method for producing high titer Sendai virus

Objective:To establish a preparative method of producing high titer Sendai virus using passage cells in empirical study so that it can supersede the one of inoculating chick embryo eggs.Methods:First of all,score of cytopatho effect(CPE) and yield were observed after three cell lines to be infected with Sendai virus.And then the cell lines sensitive to Sendai virus and the peak yield of resulting virus were determined.These data were obtained in every passage throughout three consecutive passages.Secondly,the impact of different viral adsorption time on the yield was investigated.Thirdly,a preparative method of producing high titer Sendai virus was established by comprehensive analysis.Results:The variables mentioned above showed that rat neurogliocytoma C6 was hypersensitive to Sendai virus with apparent CPE and higher yield in different cells tested.The virus titer was adsorption-time dependent.Furthermore,the optimum viral adsorption time was about 90 minutes,and the peak titer appears at 72 hours after viral adsorption and the average titer was 11 T CID50.Moreover,Sendai virus could be passaged in rat neurogliocytoma C6 stably.Conclusion:Rat neurogliocytoma C6 is confirmed to be the sensitive cell lines to Sendai virus because of apparent CPE,high virus yield.Thus it is demonstrated that the method established will paves the way for producing high titer Sendai virus and therefore elaborating the virus in vitro in the future.