MCP-1 Expression Associated with Inflammation and Biomaterials

The study of inflammation is important in understanding the reaction between biomaterials and the immune system. In the current study, human umbilical vein endothelial cells (ECV-304), cultured with PRMI-1640, were detached from cells cultured with the supernatant of macrophages which contained TNF-α and IL-1β because of stimulation by biomaterials. Cells were then put into contact with multiple biomaterials with different biocompatibilities. Levels of monocyte chemoattractant protein-1 (MCP-1) were measured. The results suggested that level of MCP-1 cultured with supernatant of macrophages was higher than PRMI-1640 in the same biomaterials. And, exposure to the positive control, Polytetrafluoroethylene, Poly-Lactic-co-Glycolic acid and American NPG alloy, resulted in high expression of MCP-1 (p< 0.001) following cytokine stimulation. MCP-1 was also significantly expressed in β-Tricalcium Phosphate and Calcium Phosphate Cement samples (p < 0.01). These data suggested that MCP-1 was activated by both cytokines and biomaterials.