A Simplified Method for the Cloning of Heteroploid and Diploid Mammalian Cells.

A method which appears a practical and easy way to produce cloned cell lines is presented, with some observations and problems to be considered when cloning. Although the method attempts to derive cell lines from single cells by taking many precautions, it cannot be definitely stated that this goal is actually accomplished. It has yet to be demonstrated satisfactorily that a single diploid cell can in fact be cloned. The method, however, definitely produces cell lines which are derived from such a reduced population that morphological differences cannot be demonstrated within a clone, but can be demonstrated between cells from different clones.