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2014•LS International Journal of Life SciencesRequires access

Antibacterial Activity and Phytochemical Screening ofOcimum sanctum Linn.(Leaves)

M. Punithavathi, J. Aranganathan, Mehrnoush Janani

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Abstract

The antibacterial effect of the leaves of Ocium sanctum Linn (Lamiaceae) was evaluated on bacterial strains like Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Salmonella typhi and Vibrio chol-erae. The in vitro antibacterial activity was studied by agar cup method and compared with the standard antibiotic, streptomycin (1000 µg). Maximum activity was exhibited by ethanolic leaves extract against B. subtilus and V. cholerae. S. aureus, Salmonella typhi and E. coli exhibited minimum activity. The different solvent extracts showed the presence of secondary metabolites like tannin, saponin, flavonoids, alkaloids, proteins and steroids. The results obtained in the present study suggest that O. sanctum Linn can be used in treating diseases caused by the test organisms.

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What this paper is about

The antibacterial effect of the leaves of Ocium sanctum Linn (Lamiaceae) was evaluated on bacterial strains like Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Salmonella typhi and Vibrio chol-erae. The in vitro antibacterial activity was studied by agar cup method and compared with the standard antibiotic, streptomycin (1000 µg). Maximum activity was exhibited by ethanolic leaves extract against B. subtilus and V. cholerae. S. aureus, Salmonella typhi and E. coli exhibited minimum activity. The different solvent extracts showed the presence of secondary metabolites like tannin, saponin, flavonoids, alkaloids, proteins and steroids. The results obtained in the present study suggest that O. sanctum Linn can be used in treating diseases caused by the test organisms.

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Available abstract

The antibacterial effect of the leaves of Ocium sanctum Linn (Lamiaceae) was evaluated on bacterial strains like Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Salmonella typhi and Vibrio chol-erae. The in vitro antibacterial activity was studied by agar cup method and compared with the standard antibiotic, streptomycin (1000 µg). Maximum activity was exhibited by ethanolic leaves extract against B. subtilus and V. cholerae. S. aureus, Salmonella typhi and E. coli exhibited minimum activity. The different solvent extracts showed the presence of secondary metabolites like tannin, saponin, flavonoids, alkaloids, proteins and steroids. The results obtained in the present study suggest that O. sanctum Linn can be used in treating diseases caused by the test organisms.

Key concepts: Salmonella typhi, Phytochemical, Ocimum, Antibacterial activity, Traditional medicine, Staphylococcus aureus, Saponin, Lamiaceae

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