2007•Optics ExpressOpen access

Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy

Kaï Hassler, Per Rigler, Hans Blom, Rudolf Rigler, Jerker Widengren, Theo Lasser

Abstract

This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines the advantages of FCS with TIRF microscopy in a way that allows for simultaneous ultra-sensitive spectroscopic measurements using a single-point detector and convenient localization of single molecules on a surface by means of parallel imaging.

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Available abstract

Key concepts: Total internal reflection fluorescence microscope, Fluorescence correlation spectroscopy, Horseradish peroxidase, Total internal reflection, Spectroscopy, Optics, Fluorescence, Fluorescence spectroscopy