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Evaluation of the stain Viablue for the rapid estimation of viable yeast cells

T.C. Hutcheson, Terry L. McKay, L. Farr, B. Seddon

Open publisher page 31 citations

Abstract

Viablue can be used as a differential viability stain for yeast cells. Differential staining using fluorescence microscopy and viability showed a good correlation (r= 0·99). For practical purposes the staining procedure takes only a few minutes and appears ideally suited to such rapid analytical procedures as the direct epifluorescent filter technique (DEFT) and automated quality control testing of foods and beverages.

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What this paper is about

Viablue can be used as a differential viability stain for yeast cells. Differential staining using fluorescence microscopy and viability showed a good correlation (r= 0·99). For practical purposes the staining procedure takes only a few minutes and appears ideally suited to such rapid analytical procedures as the direct epifluorescent filter technique (DEFT) and automated quality control testing of foods and beverages.

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Available abstract

Viablue can be used as a differential viability stain for yeast cells. Differential staining using fluorescence microscopy and viability showed a good correlation (r= 0·99). For practical purposes the staining procedure takes only a few minutes and appears ideally suited to such rapid analytical procedures as the direct epifluorescent filter technique (DEFT) and automated quality control testing of foods and beverages.

Key concepts: Differential staining, Staining, Yeast, Stain, Biology, Fluorescence microscope, Fluorescent staining, Filter paper

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