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2009•Current Protocols in MicrobiologyRequires access

Differential Staining of Bacteria: Acid Fast Stain

Jackie Reynolds, Rita B. Moyes, Donald P. Breakwell

Open publisher page 24 citations

Abstract

Abstract Acid‐fastness is an uncommon characteristic shared by the genera Mycobacterium (Section 10A) and Nocardia . Because of this feature, this stain is extremely helpful in identification of these bacteria. Although Gram positive, acid‐fast bacteria do not take the crystal violet into the wall well, appearing very light purple rather than the deep purple of normal Gram‐positive bacteria. Curr. Protoc. Microbiol. 15:A.3H.1‐A.3H.5. © 2009 by John Wiley & Sons, Inc.

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Abstract Acid‐fastness is an uncommon characteristic shared by the genera Mycobacterium (Section 10A) and Nocardia . Because of this feature, this stain is extremely helpful in identification of these bacteria. Although Gram positive, acid‐fast bacteria do not take the crystal violet into the wall well, appearing very light purple rather than the deep purple of normal Gram‐positive bacteria. Curr. Protoc. Microbiol. 15:A.3H.1‐A.3H.5. © 2009 by John Wiley & Sons, Inc.

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Available abstract

Abstract Acid‐fastness is an uncommon characteristic shared by the genera Mycobacterium (Section 10A) and Nocardia . Because of this feature, this stain is extremely helpful in identification of these bacteria. Although Gram positive, acid‐fast bacteria do not take the crystal violet into the wall well, appearing very light purple rather than the deep purple of normal Gram‐positive bacteria. Curr. Protoc. Microbiol. 15:A.3H.1‐A.3H.5. © 2009 by John Wiley & Sons, Inc.

Key concepts: Differential staining, Biology, Stain, Staining, Bacteria, Microbiology, Acid-fast, Differential (mechanical device)

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